Merck
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D9564

Sigma-Aldrich

DAPI, dilactate

≥98% (HPLC)

Synonym(s):
4′,6-Diamidino-2-phenyindole, dilactate
Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥98% (HPLC)

form

powder

solubility

H2O: 20 mg/mL

ε (extinction coefficient)

28.00-32.50 at 345-351 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells.[1][2] DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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