DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.
DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.
A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.