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77160

Sigma-Aldrich

Pepsin from porcine gastric mucosa

powder, slightly beige, ≥500 U/mg

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Synonym(s):
Pepsin A, Pepsin from hog stomach
CAS Number:
9001-75-6
Enzyme Commission number:
3.4.23.1 (BRENDA, IUBMB)
EC Number:
232-629-3
MDL number:
MFCD00081840
eCl@ss:
42010127
NACRES:
NA.54

form

powder

specific activity

≥500 U/mg

mol wt

35 kDa

color

slightly beige

UniProt accession no.

P00791

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

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Pepsin from porcine gastric mucosa powder, slightly beige, ≥500 U/mg

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77160

Pepsin from porcine gastric mucosa

Pepsin from porcine gastric mucosa powder, slightly beige, 1200-2400 U/mg

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77151

Pepsin from porcine gastric mucosa

Pepsin from porcine gastric mucosa lyophilized powder, ≥2,500 units/mg protein (E1%/280)

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P7012

Pepsin from porcine gastric mucosa

Pepsin from porcine gastric mucosa powder, ≥400 units/mg protein

Sigma-Aldrich

P7125

Pepsin from porcine gastric mucosa

specific activity

≥500 U/mg

specific activity

1200-2400 U/mg

specific activity

≥2,500 units/mg protein (E1%/280)

specific activity

≥400 units/mg protein

mol wt

35 kDa

mol wt

35 kDa

mol wt

35 kDa

mol wt

35 kDa

color

slightly beige

color

slightly beige

color

off-white to yellow

color

-

UniProt accession no.

P00791

UniProt accession no.

P00791

UniProt accession no.

P00791

UniProt accession no.

P00791

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unit Definition

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Other Notes

Peptide synthesis, Review.
Sales restrictions may apply.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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C A Abdel Malak et al.
International journal of peptide and protein research, 41(2), 97-101 (1993-02-01)
Swine pepsin at pH 5 efficiently catalyzes a condensation between Z-Ala-Ala-Phe-OH and p-nitroanilides of Leu, Phe, Val, Ala and Arg that leads to formation of corresponding benzyloxycarbonyl-tetrapeptide p-nitroanilides with yields of 70-90%. These reactions are complicated by co-precipitation of pepsin
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