Merck
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D8906

Sigma-Aldrich

Dextran sulfate sodium salt from Leuconostoc spp.

for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

CAS Number:
MDL number:
NACRES:
NA.31

biological source

synthetic

Quality Level

grade

for molecular biology

form

powder

mol wt

average Mw >500,000 (dextran starting material)

contains

0.5-2% phosphate buffer

composition

Sulfur Content, 16.0-19.0%

solubility

H2O: 100 mg/mL

storage temp.

2-8°C

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General description

Dextran sulfates are supplied as the sodium salt forms that are soluble and stable in water. It consist of 17% (approx.) sulfur equivalent to 2.3 (approx.) sulfate groups per glucosyl residue. Dextran is a (complex branched glucan) polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages and the remaining (163) linkages account for the branching of dextran.

Application

Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Packaging

5, 10, 50, 100, 500 g in poly bottle

Suitability

Tested for suitability in nucleic acid hybridizations

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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