A4327

Albumin from human serum

lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

• Synonym(s): HSA
• CAS Number: 70024-90-7
• EC Number: 274-272-6
• MDL number: MFCD00081418
• NACRES: NA.25

Properties

• biological source: human
• Quality Level: 200
• Assay: ≥96% (agarose gel electrophoresis)
• form: lyophilized powder
• mol wt: monomer calculated mol wt 66478 Da
• technique(s): tissue culture: suitable
• impurities: HIV I and HIVII, HCV and HBsAg, tested negative
• solubility: H₂O: soluble 50 mg/mL
• UniProt accession no.: P02768
• foreign activity: Alkaline phosphatase ≤0.001 units/mg protein; Peroxidase ≤0.001 units/mg protein; Protease ≤0.0001 units/mg protein
• storage temp.: 2-8°C
• InChI: 1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11
• InChI key: QYSGYZVSCZSLHT-UHFFFAOYSA-N
• Gene Information: human ... ALB(213)

Description

General description

Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties:

• Essentially protease-free / low protease content
• Low alkaline phosphatase content
• Low peroxidase content

Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest.

Protease-free HSA is especially useful for applications like:

• Enzyme assays
• Protein-based assays
• Protease-sensitive techniques such as:

(a) Enzyme immunoassay (EIA)
(b) Nucleic acid hybridization
(c) Radioimmunoassay (RIA)

Application

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.
It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing.

Biochem/physiol Actions

Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.

Features and Benefits

• Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein)
• Low alkaline phosphatase activity (≤ 0.001 units/mg protein)
• Low peroxidase activity (≤ 0.001 units/mg protein)

Preparation Note

Prepared from A1653

Other Notes

View more information on human serum albumin.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves