A8706

Avidin from egg white

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

• Synonym(s): AVID_CHICK
• CAS Number: 1405-69-2
• EC Number: 215-783-6
• MDL number: MFCD00130572
• NACRES: NA.56

Properties

• recombinant: expressed in corn
• Quality Level: 200
• Assay: ≥80% protein basis (biuret)
• form: powder
• specific activity: ≥12 units/mg protein (E1%/280)
• mol wt: glycoprotein 66 kDa; subunit 16 kDa
• technique(s): ELISA: suitable; immunoblotting: suitable; immunoelectrophoresis: suitable
• UniProt accession no.: P02701
• storage temp.: 2-8°C
• Gene Information: chicken ... AVD(396260)

Description

General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Avidin forms an extremely strong complex with d-biotin (K_d ~ 10^-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves