The specific salmon species is not determined for this product. See the link below to review a sample Certificate of Origin for this material:
https://www.sigmaaldrich.com/coo/undefined/D9156/SLBM7249V.
D9156
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Synonym(s):
single-stranded template DNA
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1 ML
PLN 434.00
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PLN 1,290.00
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PLN 1,440.00
PLN 434.00
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About This Item
Assay:
9-11 mg/mL (DNA concentration)
Biological source:
fish testis (salmon)
Form:
solution
Storage temp.:
−20°C
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biological source
fish testis (salmon)
grade
Molecular Biology
assay
9-11 mg/mL (DNA concentration)
form
solution
concentration
10 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| grade for molecular biology | grade for molecular biology, puriss. p.a. | grade for molecular biology | grade for molecular biology |
| biological source fish testis (salmon) | biological source - | biological source - | biological source bovine thymus |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 200 |
| form solution | form lyophilized powder | form solution | form solution |
| assay 9-11 mg/mL (DNA concentration) | assay - | assay 9-11 mg/mL | assay 9-11 mg/mL (DNA concentration) |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in dry ice |
General description
A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .
Application
Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples[1] and human blood monocyte subpopulations.[2]
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Preparation Note
This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
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H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
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For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for
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Protocols
Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
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Instructions
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