E4011

ExtrAvidin^®−R-Phycoerythrin

buffered aqueous solution

• MDL number: MFCD00163600
• NACRES: NA.46

Properties

• conjugate: phycoerythrin (R-PE) conjugate
• Quality Level: 200
• form: buffered aqueous solution
• storage condition: protect from light
• extent of labeling: 1-4 mol PE per mol ExtrAvidin^®
• technique(s): flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues
• storage temp.: 2-8°C

Description

General description

ExtrAvidin is a chemically modified protein purified from egg whites.
The extravidin-R-phycoerythrin protein can be conjugated with fluorescein-labelled arylacetamide, a κ opioid ligand and biotin-conjugated anti-fluorescein IgG to identify the subpopulations of thymocytes that express κ opioid receptor. This product has shown specificity for rabbit anti-avidin in immunoelectrophoresis analysis.

ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Immunogen

Thymocytes present in C57BL mice.

Application

ExtrAvidin^®-R-Phycoerythrin has been used

• In flow cytometric analysis to confirm membrane expression of ncamp-1 (cationic oligodeoxynucleotide binding protein).
• In indirect immunofluorescence.
• In Western blotting.

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.

Packaging

1 mL in glass bottle

100 test

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable