Merck
All Photos(1)

F2426

Millipore

EZview Red ANTI-FLAG® M2 Affinity Gel

clone M2

Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

clone

M2, monoclonal

Quality Level

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3xFLAG® tag, DYKDDDDK tag)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

4% agarose bead; 45-165μm bead size

isotype

IgG1

capacity

≥0.6 mg/mL, gel binding capacity

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.

Specificity

Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.

Application

Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.

Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide

Browse additional application references in our FLAG® Literature portal.

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service