GE17-1166-01

HiPrep^™ 16/60 Sephacryl^® S-200 HR

Cytiva 17-1166-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

• NACRES: SB.52

Properties

• material: polypropylene
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• mol wt: 5000-250000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 17-1166-01
• parameter: <1 mL/min flow rate; <5 mL sample volume; 0.15 Mpa (22 psi); 22 psi
• bed volume: 120 mL
• column L × I.D.: 60 cm × 16 mm
• matrix: dextran-acrylamide particle platform; allyl dextran and N, N’-methylenebisacrylamide; polymeric
• matrix active group: dextran-acrylamide phase
• avg. part. size: 50 μm
• cleaning: 2-13
• working range: 3-11
• separation technique: size exclusion (SEC)

Description

General description

HiPrep^™ 16/60 Sephacryl^® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl^® S-200 is used for separating midsize proteins including antibodies and serum proteins.

Features and Benefits

• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
• Gel filtration columns for low to medium pressure AKTA systems.
• Well-established in industrial production.
• The HiPrep^™ Sephacryl^® series offers reproducible separations over a wide molecular weight range.
• Convenient, prepacked HiPrep^™ Sephacryl^® columns speed up protein purification.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)

Gel filtration columns for low to medium pressure ÄKTA systems.

for preparative separation of biomolecules of up to 5 mL sample volume

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva

Sephacryl is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids