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HiPrep 16/60 Sephacryl® S-200 HR

Cytiva 17-1166-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size




shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

5000-250000 Da (globular proteins)


pkg of 1 ea


Cytiva 17-1166-01


<1 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)
22 psi

bed volume

120 mL

column L × I.D.

60 cm × 16 mm


dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm



working range


separation technique

size exclusion (SEC)

Related Categories

General description

HiPrep 16/60 Sephacryl® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-200 is used for separating midsize proteins including antibodies and serum proteins.

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.


All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit

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Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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