GE17-5174-01

Superdex^® 75 10/300 GL

Cytiva 17-5174-01, column L × I.D. 30 cm × 10 mm, 13 μm avg. part. size

• NACRES: SB.52

Properties

• material: bed support (polyamide); glass column; polypropylene fiber (reinforced glass)
• product line: Superdex^®
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• mol wt: 3000-70000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 17-5174-01
• parameter: 0.5 ml/min - 1 ml/min flow rate; 1.8 Mpa (261 psi); 25-500 μL sample volume
• bed volume: 24 mL
• column L × I.D.: 30 cm × 10 mm
• matrix: agarose-dextran composite; polymeric
• avg. part. size: 13 μm
• cleaning: 1-14
• working range: 3-12
• separation technique: size exclusion (SEC)

Description

General description

Superdex^® 75 10/300 GL is a prepacked gel filtration column for high-resolution, semipreparative, and analytical separations of biomolecules. Superdex^® 75 has a separation range for molecules with molecular weights between 3 000 and 70 000.

Superdex^® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.

Superdex^® 75 5/150 GL and Superdex^® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.

Superdex^® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family excellent reproducibility and durability in purification using a chromatography system.

Features and Benefits

• High-resolution separations of proteins, peptides, and other biomolecules according to size.
• Well suited for the polishing step in a purification procedure.
• Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
• Easy and predictable scale-up.
• Excellent reproducibility and durability.
• For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)

for high-resolution, semipreperative and analytical separations of biomolecules

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

Superdex is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids