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HisTrap Fast Flow

Cytiva 17-5255-01, pack of 5 × 5 mL


shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.


pack of 5 × 5 mL


Cytiva 17-5255-01


<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm


6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm


2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)


~40 mg binding capacity(histidine-tagged protein)


suitable for bioprocess medium

General description

HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose® 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose® 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Features and Benefits

  • High binding capacity, approx. 40 mg/mL medium.
  • Negligible leakage of Ni2+.
  • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit

Legal Information

HiTrap is a registered trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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