GE28-9893-31

HiLoad^® 16/600 Superdex^® 30 pg

Cytiva 28-9893-31, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

• NACRES: SB.52

Properties

• material: bed support (polyamide); glass column; polypropylene fiber (reinforced glass)
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• mol wt: <10000 Da (globular proteins)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9893-31
• parameter: <1.0 mL/min flow rate; <5 mL sample volume; 0.3 Mpa (42 psi)
• bed volume: 120 mL
• column L × I.D.: 60 cm × 16 mm
• matrix: agarose dextran composite; dextran-agarose particle platform; polymeric
• matrix active group: dextran-agarose phase; dextran-agarose phase
• avg. part. size: 34 μm
• cleaning: 1-14
• working range: 3-12
• separation technique: size exclusion (SEC)

Description

General description

HiLoad^® 16/600 Superdex^® 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.

Application

Superdex^® prep grade (pg) is produced by covalent bonding of Dextran to highly cross-linked Agarose.The separation properties of these media are determined by the Dextran component. Steep selectivity curves give excellent resolving power for peptides and Proteins in the molecular weight ranges, Mr < 10 000 (Superdex^® 30 pg), Mr 3 000 to 70 000 (Superdex^® 75 pg), and Mr 10 000 600 000 (Superdex^® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Features and Benefits

• High resolution with short run times and good recovery.
• High capacity at high flow rates.
• Separations can be scaled up to production levels.
• Prepacked for convenience and reproducibility.
• 1/16” fittings for convenient connections to ÄKTA^® design systems.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

for high-resolution preparative gel filtration chromatography separating peptides

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiLoad is a registered trademark of Cytiva

Superdex is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids