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HiLoad® 16/600 Superdex® 30 pg

Cytiva 28-9893-31, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size



bed support (polyamide)
glass column
polypropylene fiber (reinforced glass)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

mol wt

<10000 Da (globular proteins)


pkg of 1 ea


Cytiva 28-9893-31


<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

bed volume

120 mL

column L × I.D.

60 cm × 16 mm


agarose dextran composite
dextran-agarose particle platform

matrix active group

dextran-agarose phase
dextran-agarose phase

avg. part. size

34 μm



working range


separation technique

size exclusion (SEC)

Related Categories

General description

HiLoad® 16/600 Superdex® 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.


Superdex® prep grade (pg) is produced by covalent bonding of Dextran to highly cross-linked Agarose.The separation properties of these media are determined by the Dextran component. Steep selectivity curves give excellent resolving power for peptides and Proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Features and Benefits

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to ÄKTA® design systems.


All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating peptides

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit

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Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva



Signal Word


Hazard Statements

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

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Certificate of Origin

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