Merck
All Photos(1)

GE29-0915-96

Sigma-Aldrich

Superose® 6 Increase 10/300 GL

Cytiva 29-0915-96, 10/300 GL

Synonym(s):
Superose® 6 Increase
NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superose®

mol wt

5000-5,000,000 Da (globular proteins)

packaging

package of 1 ea

manufacturer/tradename

Cytiva 29-0915-96

parameter

<0.35 mL/min flow rate
<0.75 mL/min flow rate (20% ethanol at room temp)
<0.75 mL/min flow rate
<1.50 mL/min flow rate (water at room temp)
3 Mpa

bed volume

24 mL

sample volume

25-500 μL

matrix

cross-linked agarose
polymeric

matrix active group

agarose phase

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

Looking for similar products? Visit Product Comparison Guide

General description

Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Suitability

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

Storage Class Code

3 - Flammable liquids

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service