I4401
Interferon-αA/D human
≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Synonym(s):
IFN-αA/D
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100000 UNITS
PLN 2,370.00
PLN 2,370.00
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biological source
human
recombinant
expressed in E. coli
assay
≥95%
form
buffered aqueous solution
mol wt
19 kDa
packaging
pkg of 100K units
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
colorless
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... IFNA2(3440)
1 of 4
This Item | I4276 | H6166 | I8657 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥95% | assay ≥95% | assay ≥95% (SDS-PAGE) | assay ≥95% |
| biological source human | biological source human | biological source human | biological source rat |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in HEK 293 cells | recombinant expressed in bacteria |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
Analysis Note
The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV).[1][2] A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.[3]
Application
Interferon-αA/D human has been used:
- for identification of coronavirus replicase inhibitors[4]
- in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication[5]
- in combination with vitamin D to check reduction in HCV protein production in cell culture[6]
Biochem/physiol Actions
Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.
This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.
Physical form
Solution in phosphate buffered saline containing 0.1% bovine serum albumin
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Hector Moreno et al.
Viruses, 13(7) (2021-08-11)
The New World (NW) mammarenavirus group includes several zoonotic highly pathogenic viruses, such as Junin (JUNV) or Machupo (MACV). Contrary to the Old World mammarenavirus group, these viruses are not able to completely suppress the innate immune response and trigger
Sophie Rogée et al.
Antiviral research, 161, 1-9 (2018-10-28)
Rabies virus transmits from animals to humans and causes encephalitis. Every year more than 15 million people receive a post exposure prophylaxis (PEP) treatment that is highly effective in the prevention of rabies disease. However, when clinical symptoms appear, for
Guido Wollmann et al.
Journal of virology, 89(13), 6711-6724 (2015-04-17)
High-grade tumors in the brain are among the deadliest of cancers. Here, we took a promising oncolytic virus, vesicular stomatitis virus (VSV), and tested the hypothesis that the neurotoxicity associated with the virus could be eliminated without blocking its oncolytic
Julio A Gutierrez et al.
Journal of virology & antiviral research, 3(3), doi:10-doi:10 (2015-11-26)
Previous studies suggest that low serum 25-hydroxyvitamin D [25(OH) D] levels are associated with reduced responsiveness to interferon and ribavirin therapy. We investigated the impact of vitamin D metabolites on HCV and cellular gene expression in cultured hepatoma cells. HCV
David L Wyles et al.
Antimicrobial agents and chemotherapy, 52(5), 1862-1864 (2008-03-12)
Rapid emergence of resistance to monotherapy with virus-specific inhibitors necessitates combination therapy. ACH-806 is a hepatitis C virus NS4A inhibitor with a novel mechanism of action and resistance pathway. This compound was synergistic with NS3 protease inhibitors and NS5B nucleoside
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