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I4777

Sigma-Aldrich

Interferon-γ from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
IFN-γ
MDL number:
MFCD00131391
NACRES:
NA.32

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

mol wt

protein 15.6 kDa

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

solubility

water: soluble 1.000 mL, clear, colorless

UniProt accession no.

P01580

storage temp.

−20°C

Gene Information

mouse ... Ifng(15978)

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I4777

Interferon-γ from mouse

Interferon-γ from rat ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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I3275

Interferon-γ from rat

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I8657

Interferon α from rat

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I9032

Interferon β from mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in bacteria

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥95%

assay

≥95%

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

mol wt

protein 15.6 kDa

mol wt

15.5 kDa

mol wt

~19 kDa

mol wt

~20 kDa

packaging

pkg of 0.1 mg

packaging

pkg of 0.1 mg

packaging

pkg of 1 vial

packaging

pkg of 1 vial

Application

Interferon-γ from mouse has been used:
  • for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c+ (CD11 antigen-like family member C) lung macrophages
  • for stimulation of murine macrophages to induce the production of nitrite and nitrate
  • to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation

Biochem/physiol Actions

Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.

Analysis Note

The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Sergio Arias-Negrete et al.
Analytical biochemistry, 328(1), 14-21 (2004-04-15)
Preparation of a nitrate reductase lysate of Escherichia coli MC1061 to measure nitrate and nitrite in biologic fluids is described. To obtain the crude bacterial lysate containing nitrate reductase activity, E. coli MC1061 was subjected to 16-20 freeze-thawing cycles, from
S N Vogel et al.
Infection and immunity, 38(2), 681-685 (1982-11-01)
Silica has been used for many years as an agent which selectively alters macrophage functions and, as such, has been used to assess the role of macrophages in the immune response to a variety of microbial and chemically defined agents.
P W Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(19), 5842-5846 (1983-10-01)
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of
Tatiana Cheusova et al.
Genes & development, 20(13), 1800-1816 (2006-07-05)
The release of Agrin by motoneurons activates the muscle-specific receptor tyrosine kinase (MuSK) as the main organizer of subsynaptic specializations at the neuromuscular junction. MuSK downstream signaling is largely undefined. Here we show that protein kinase CK2 interacts and colocalizes
Marta Pulido-Salgado et al.
Scientific reports, 8(1), 16096-16096 (2018-11-02)
Microglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with
View All Related Papers

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