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I8782

Sigma-Aldrich

Interferon αA from mouse

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

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Synonym(s):
IFN-αA
MDL number:
MFCD02253032
NACRES:
NA.32

biological source

mouse

Quality Level

200

recombinant

expressed in E. coli

Assay

≥95%

form

buffered aqueous solution

mol wt

20 kDa

technique(s)

cell culture | mammalian: suitable

color

colorless

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... Ifna3(110218)

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Interferon-α A Protein, Recombinant mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥95%

assay

≥95%

assay

≥95%

assay

>95%

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

liquid

mol wt

20 kDa

mol wt

~20 kDa

mol wt

19 kDa

mol wt

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Biochem/physiol Actions

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities. Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes) and ruminant embryos (τ subtypes). Interferon induces a variety of effects on target cells including antiviral, antiproliferative and immunomodulatory activities.

Packaging

Minimum 1x105 units/vial.

Physical form

Solution in phosphate buffered saline containing 0.1% bovine serum albumin.

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon α.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Interferon standards and general abbreviations.
S Pestka
Methods in enzymology, 119, 14-23 (1986-01-01)
S Rubinstein et al.
Journal of virology, 37(2), 755-758 (1981-02-01)
A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed
A convenient and rapid cytopathic effect inhibition assay for interferon.
P C Familletti et al.
Methods in enzymology, 78(Pt A), 387-394 (1981-01-01)

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