I9032
Interferon β from mouse
≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Synonym(s):
IFN β
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1 VIAL
PLN 4,810.00
PLN 4,810.00
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biological source
mouse
recombinant
expressed in E. coli
assay
≥95%
form
buffered aqueous solution
mol wt
~20 kDa
packaging
pkg of 1 vial
concentration
2560000 units/mL
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
mouse ... Ifnb1(15977)
1 of 4
This Item | I8657 | IF005 | IF011 |
|---|---|---|---|
| biological source mouse | biological source rat | biological source mouse | biological source mouse |
| assay ≥95% | assay ≥95% | assay >98% (SDS-PAGE and HPLC) | assay ≥95% |
| recombinant expressed in E. coli | recombinant expressed in bacteria | recombinant - | recombinant expressed in E. coli |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form buffered aqueous solution | form lyophilized powder | form liquid | form liquid |
Analysis Note
The biological activity is determined in the cytopathic effect inhibition assay using mouse L929 cells with encephalomyocarditis virus. The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for mouse interferon β.
Application
Interferon β from mouse has been used:
- to induce the activation of cholesterol 25-hydroxylase, by in vitro stimulation of bone marrow-derived macrophages, dendritic cells and CD11c+ lung macrophages[1]
- to stimulate splenocytes, in order to study the secretion and regulation of IFN-γ [2]
- to intranasally pre-treat neonatal mice, to study its effects on the protective actions of Lactobacillus rhamnosus GG (LGG)[3]
- as a standard to treat MEF-Mx2-luc-BKO reporter cell line for IFN-β measurement, in response to human adenovirus-5 (HAdV-C5)[4]
Biochem/physiol Actions
Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.
In presence of virus infection, IFN β (interferon β) is upregulated and plays a crucial role in innate antiviral response.[7] It is also important for anti-inflammatory effects.[8]
Interferon β (IFN-β) suppresses a variety of proinflammatory cytokines in lymphocytes including the interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α).[9] It also favors the synthesis of antagonists associated with interleukin IL-10 and IL-1 receptor.[9] IFN-β downregulates interferon γ production but enhances transforming growth factor β1 expression.[9] It has therapeutic potential in treating rheumatoid arthritis[9] and relapsing-remitting multiple sclerosis (MS).[10]
General description
IFN β (interferon β) is from the type 1 interferon family of cytokines.[8]
Interferon β (IFN-β) possesses a signal peptide region. It has comparatively more hydrophobic residues and displays 30% homology with IFN-α.[13]
The gene encoding interferon β (Infb1) is localized on mouse chromosome 4.[14]
Physical form
Solution in 20 mM HEPES, pH 6.0, 0.5 M NaCl, 6% glycerol, and 0.1% bovine serum albumin.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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ENU-induced mutation in USP18 causes hyperactivation of IFN-alphabeta signalling and suppresses STAT4-induced IFN-gamma production resulting in increased susceptibility to Salmonella Typhimurium
Richer E, et al.
Journal of immunology (Baltimore, Md. : 1950), 185(6), 3593-3593 (2010)
P Keskinen et al.
Immunology, 91(3), 421-429 (1997-07-01)
HLA class I and II molecules play a central role in regulating host immune responses against microbial infections because they present foreign antigens to CD8+ and CD4+ T lymphocytes, respectively. Many cytokines, especially interferons (IFN), are known to upregulate human
Nanthapon Ruangkiattikul et al.
Virulence, 8(7), 1303-1315 (2017-04-20)
Type I interferons (IFN-I), such as IFN-α and IFN-β are important messengers in the host response against bacterial infections. Knowledge about the role of IFN-I in infections by nontuberculous mycobacteria (NTM) is limited. Here we show that macrophages infected with
Manuel Hagen et al.
Cell cycle (Georgetown, Tex.), 15(14), 1929-1937 (2016-05-28)
Podocytes are terminally differentiated renal cells, lacking the ability to regenerate by proliferation. However, during renal injury, podocytes re-enter into the cell cycle but fail to divide. Earlier studies suggested that re-entry into cell cycle results in loss of podocytes
Patrick Ejlerskov et al.
Cell, 163(2), 324-339 (2015-10-10)
Neurodegenerative diseases have been linked to inflammation, but whether altered immunomodulation plays a causative role in neurodegeneration is not clear. We show that lack of cytokine interferon-β (IFN-β) signaling causes spontaneous neurodegeneration in the absence of neurodegenerative disease-causing mutant proteins.
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