L2164

Anti-Luciferase antibody, Mouse monoclonal

clone LUC-1, purified from hybridoma cell culture

• MDL number: MFCD01092407
• NACRES: NA.56

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: LUC-1, monoclonal
• form: buffered aqueous solution
• mol wt: 60 kDa
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: 20-40 μg/mL using transfected 293Tcells expressing luciferase; immunocytochemistry: suitable; western blot: 2-4 μg/mL using whole extract of transfected 293T cells expressing luciferase; western blot: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other.

The mouse monoclonal Anti-Luciferase antibody recognizes the recombinant luciferase in transfected cells. This antibody provides user with an alternative detection method for luciferase. Since it directly detects the luciferase protein, it is not dependent on either the luciferase activity or the measeurement of rapid reaction kinetics. This also allows for the detection of the luciferase enzyme expression in situ with reproducibility between sample sets.

Specificity

Anti-Luciferase antibody, Mouse monoclonal recognizes recombinant luciferase in transfected eukaryotic (293T) cells.

Application

Anti-Luciferase antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg²⁺, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Working Dilution: 1-2 μg/ml

Storage and Stability

For continuous use store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in a frost-free freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable