L4391

Lipopolysaccharides from Escherichia coli O111:B4

γ-irradiated, BioXtra, suitable for cell culture

• Synonym(s): LPS
• EC Number: 297-473-0
• MDL number: MFCD00164401
• NACRES: NA.75

Properties

• biological source: Escherichia coli (O111:B4)
• Quality Level: 300
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• purified by: gel-filtration chromatography
• storage condition: desiccated
• technique(s): cell culture | mammalian: suitable
• impurities: <1% Protein (Lowry)
• solubility: H₂O: 5 mg/mL, slightly hazy
• storage temp.: 2-8°C

Description

General description

Lipopolysaccharides (LPS) are intricate molecules consisting of lipids and polysaccharides, predominantly present in the outer membrane of Gram-negative bacteria. Comprising lipid A, the core oligosaccharide (core), and the O-specific polysaccharide or O antigen (OAg), LPS is anchored to the outer bacterial membrane, with lipid A primarily responsible for its associated toxicity. Functioning as a potent activator of the proinflammatory response, LPS engages various mammalian cells, including macrophages, monocytes, and endothelial cells. While serving a protective role for Gram-negative bacteria against environmental stress, LPS also triggers recognition by the innate immune system during infections.

Application

This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Features and Benefits

• High-purity compound suitable for a wide variety of research applications

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable