L9420

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×10¹⁰ units/mg protein

• Synonym(s): Luciferase firefly
• CAS Number: 61970-00-1
• Enzyme Commission number: 1.13.12.7 (BRENDA, IUBMB)
• MDL number: MFCD00131546

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥98% (SDS-PAGE)
• form: buffered aqueous solution
• specific activity: ≥10×10¹⁰ units/mg protein
• mol wt: 62 kDa
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Application

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Unit Definition

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of ¹⁴C in a PPO/POPOP cocktail.

Physical form

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H302 - H373
• Precautionary Statements: P260 - P264 - P270 - P301 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Oral - STOT RE 2 Oral
• Target Organs: Kidney
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 231.8 °F
• Flash Point(C): 111 °C