M8316

Anti-MRP2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-ABCC2, Anti-Multidrug Resistance Associated Protein 2, Anti-cMOAT, Anti-cMRP
• MDL number: MFCD01865475
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~190 kDa
• species reactivity: rat, human
• technique(s): indirect immunofluorescence: 1:100 using paraformaldehyde-fixed HepG2 cells; indirect immunofluorescence: 1:100 using rat liver frozen sections; western blot: 1:1,000 using whole extract of HepG2 human hepatoblastoma cells
• UniProt accession no.: Q92887
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... ABCC2(1244) ; rat ... Abcc2(25303)

Description

General description

Multidrug Resistance-associated Protein 2 (MRP2), also called as canalicular Multispecific Organic Anion Transporter (cMOAT), is a member of the CFTR/MRP (ABCC) subfamily of the large ATP-Binding Cassette (ABC) transporter family of transmembrane proteins. MRP2 is normally expressed in the liver, gallbladder, kidney proximal tubules, placenta, duodenum and small intestine. Localization pattern appears to vary in different species. MRP2 is predominantly localized to the apical membrane of polarized cells.

Multidrug resistance associated protein 2 (MRP2) is expressed in endothelial cells of the blood-brain barrier, various carcinoma cell lines and tumors. The gene encoding MRP2 is localized on human chromosome 10q24 and consists of 32 exons.

Specificity

Additional lower bands including an approx. 175 kDa band representing an immature unglycosylated form may be detected in various extract preparations.

Immunogen

synthetic C-terminal peptide corresponding to amino acids 1528-1545 of human MRP2 conjugated to KLH.

Application

Anti-MRP2 antibody produced in rabbit has been used in:

• immunoblotting
• western blotting
• immunofluorescence

Biochem/physiol Actions

Multidrug Resistance-associated Protein 2 (MRP2) transports endogenous and exogenous anionic conjugates from hepatocytes to the bile. Thus, it contributes to bile flow and plays a role in detoxification and defense against oxidative stress. Patients with the rare autosomal recessive Dubin-Johnson Syndrome develop a mild liver disease caused by MRP2 deficiency. Up regulation of MRP2 expression may be found in hepatocellular carcinomas.

Multidrug resistance associated protein 2 (MRP2) is a transporter which is involved in the efflux of various xenobiotic compounds like doxorubicin and methotrexate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable