Merck
All Photos(1)

MAK113

Sigma-Aldrich

ATPase/GTPase Activity Assay Kit

sufficient for 200 colorimetric tests

All Photos(1)

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

storage temp.

2-8°C

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ATPase/GTPase Activity Assay Kit sufficient for 200 colorimetric tests

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MAK113

ATPase/GTPase Activity Assay Kit

ADP/ATP Ratio Assay Kit sufficient for 100 tests (bioluminescent)

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MAK135

ADP/ATP Ratio Assay Kit

GAPDH Activity Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK277

GAPDH Activity Assay Kit

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK015

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit

usage

sufficient for 200 colorimetric tests

usage

sufficient for 100 tests (bioluminescent)

usage

-

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

detection method

chemiluminescent

detection method

colorimetric

detection method

colorimetric

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.

Principle

The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 1

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