N7653

p-Nitrophenyl Phosphate Liquid Substrate System

liquid

• Synonym(s): 4-Nitrophenyl phosphate disodium salt solution, pNPP
• Linear Formula: O₂NC₆H₄OP(O)(ONa)₂
• CAS Number: 4264-83-9
• Molecular Weight: 263.05
• MDL number: MFCD00007319
• PubChem Substance ID: 24897835
• NACRES: NA.83

Properties

• form: liquid
• Quality Level: 200
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChI key: VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.

Packaging

100 mL in glass bottle

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Caution

Stable at least one year when stored below 0 °C.

Physical form

Ready-to-use.

Safety Information

• Pictograms: GHS05,GHS08
• Signal Word: Danger
• Hazard Statements: H290 - H315 - H318 - H361fd - H373
• Precautionary Statements: P202 - P234 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable