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N8271

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

recombinant, expressed in E. coli, buffered aqueous solution

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Synonym(s):
Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase
CAS Number:
9001-67-6
Enzyme Commission number:
3.2.1.18 (BRENDA, IUBMB)
MDL number:
MFCD00131711
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

form

buffered aqueous solution

specific activity

≥135 units/mg protein

mol wt

88 kDa
 95 kDa

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

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This Item
N3786480716N6514
Sigma-Aldrich

Sigma-Aldrich

N8271

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens Proteomics Grade, suitable for MALDI-TOF MS

Sigma-Aldrich

N3786

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Sigma-Aldrich

Sigma-Aldrich

480716

α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli

Neuraminidase from Vibrio cholerae Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

Sigma-Aldrich

N6514

Neuraminidase from Vibrio cholerae

form

buffered aqueous solution

form

lyophilized powder

form

liquid

form

buffered aqueous solution

specific activity

≥135 units/mg protein

specific activity

≥25 U/vial

specific activity

≥40 units/mg protein, ≥5 units/mL

specific activity

8-24 units/mg protein (Lowry, using NAN-lactose)

mol wt

88 kDa, 95 kDa

mol wt

-

mol wt

-

mol wt

-

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

foreign activity

-

foreign activity

N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected

foreign activity

Protease and NAN-aldolase, present

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Unit Definition

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Preparation Note

Expressed in glycosidase-free hosts.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
Audu J Natala et al.
Journal of medical entomology, 50(1), 85-93 (2013-02-23)
Amblyomma variegatum F. are obligate hematophagous ectoparasites of livestock that serve as the vectors of Ehrlichia ruminantium (formerly known as Cowdria ruminantium), the causative agent of heartwater disease. In the light of the fact that they are blood-feeding, their salivary
Dominic Meusch et al.
Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
Jin-Hyo Kim et al.
Science (New York, N.Y.), 340(6128), 71-75 (2013-02-23)
Influenza antiviral agents play important roles in modulating disease severity and in controlling pandemics while vaccines are prepared, but the development of resistance to agents like the commonly used neuraminidase inhibitor oseltamivir may limit their future utility. We report here
Charng-Sheng Tsai et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(7), 2466-2471 (2013-01-30)
Alkyne-hinged 3-fluorosialyl fluoride (DFSA) containing an alkyne group was shown to be a mechanism-based target-specific irreversible inhibitor of sialidases. The ester-protected analog DFSA (PDFSA) is a membrane-permeable precursor of DFSA designed to be used in living cells, and it was
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