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O3008

Sigma-Aldrich

Oleic Acid-Albumin from bovine serum

liquid, sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
Oleic acid-BSA complex
MDL number:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

composition

Oleic acid, 2 mol/mol albumin

concentration

100 mg/mL BSA in DPBS
90-120 mg/mL protein (biuret)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

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This Item
L9530A4161A1470
Bovine Serum Albumin lyophilized powder, essentially globulin free, BioReagent, suitable for cell culture

Sigma-Aldrich

A4161

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, low endotoxin, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A1470

Bovine Serum Albumin

form

liquid

form

liquid

form

lyophilized powder

form

lyophilized powder

concentration

100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)

concentration

100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

≤400 EU/mL endotoxin

impurities

essentially globulin free

impurities

≤20 ppm Pb, ≤3 EU/mg endotoxin

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Oleic Acid-Albumin from bovine serum has been used: as a component of the Dulbecco′s modified Eagle′s medium (DMEM) to study adipogenic differentiation in chicken embryonic fibroblast cell lines, to study its effects on benign prostate epithelial cells as a component of the cell culture medium to quantify triglycerides in human epithelial colorectal adenocarcinoma cells

Biochem/physiol Actions

Fatty Acid-Albumin - complexes are used as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Serum proteins may help in the release of beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) shows a positive effect on the growth of various cells.

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Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Fatty acids of the n-3, n-6 and n-9 families are important supplements for cell culture systems. They are important in cell culture systems used to biomanufacture heterologous proteins, such as monoclonal antibodies.

How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

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