R1386
RNA Sample Loading Buffer
for NA electrophoresis, without ethidium bromide
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5 VIALS
PLN 495.00
PLN 495.00
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About This Item
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grade
Molecular Biology
form
solution
foreign activity
RNase, none detected
storage temp.
−20°C
Quality Level
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This Item | R4268 | G2526 | G7654 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form solution | form liquid | form liquid | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. 2-8°C |
| foreign activity RNase, none detected | foreign activity RNase, none detected | foreign activity RNAse, none detected | foreign activity - |
Application
RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.
Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.[1]
General description
RNA loading buffer is used as a tracking dye during RNA electrophoresis. The dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.
Other Notes
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
Preparation Note
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.
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