R4018

Ribonucleic acid, transfer, phenylalanine specific from brewer's yeast

• Synonym(s): Transfer RNA, Yeast Phe tRNA, tRNA
• MDL number: MFCD00132200
• NACRES: NA.55

Properties

• Quality Level: 100
• grade: for molecular biology
• form: lyophilized powder
• solubility: water: 2 mg/mL
• storage temp.: 2-8°C

Description

General description

Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.

The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.

The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.

This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.

Application

Suitable for use:

• As a tRNA standard
• As a substrate in tRNA synthatase assays
• As a substrate in other reactions involving tRNAs

Packaging

1, 5 mg

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Preparation Note

Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves