The volume is approximately 0.276 mL per vial which equates to roughly 1.38 mL total volume for all 5 vials. The number of samples that can be processed is dependent on the sample size. The protocol suggests adding 1 volume of sample to 2-5 volumes of RNA Sample Loading Buffer. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/866/807/r4268dat.pdf
R4268
RNA Sample Loading Buffer
for NA electrophoresis, with ethidium bromide (50 μg/mL)
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5 VIALS
PLN 517.00
PLN 517.00
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Product Name
RNA Sample Loading Buffer, for NA electrophoresis, with ethidium bromide (50 μg/mL)
grade
Molecular Biology
form
liquid
concentration
1.25 ×
technique(s)
electrophoresis: suitable
foreign activity
RNase, none detected
storage temp.
−20°C
−20°C
Quality Level
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1 of 4
This Item | G2526 | R1386 | G7654 |
|---|---|---|---|
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| concentration 1.25 × | concentration - | concentration - | concentration 6 × |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form solution | form solution |
| storage temp. −20°C | storage temp. room temp | storage temp. −20°C | storage temp. 2-8°C |
Application
RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.
General description
RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.
Other Notes
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
Preparation Note
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.
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Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Carc. 1B - Muta. 2 - Repr. 1B - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Blood
Storage Class
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
75-12-7
CAS No.
75-12-7
CAS No.
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