• R5636
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Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

buffered aqueous solution

Transfer RNA, tRNA
CAS Number:
MDL number:


for molecular biology

Quality Level


buffered aqueous solution


≥9 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.


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General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.


Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
  • in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
  • as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
  • as a carrier to enhance recovery of RNA from small numbers of cells


1, 1 mL

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.


tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

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