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R6148

Sigma-Aldrich

RNase A solution

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Synonym(s):
GenElute RNAse A
NACRES:
NA.52

biological source

bovine pancreas

Quality Level

form

liquid

specific activity

1500 units/mL
3000 units/mL

concentration

20 mg/mL in Tris buffer

technique(s)

DNA purification: suitable

storage temp.

room temp

Gene Information

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This Item
R5503R464211119915001
Sigma-Aldrich

Sigma-Aldrich

R6148

RNase A solution

RNase, DNase-free from bovine pancreas

Roche

11119915001

RNase, DNase-free

form

liquid

form

lyophilized powder

form

(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

form

solution

specific activity

1500 units/mL, 3000 units/mL

specific activity

50-100 Kunitz units/mg protein

specific activity

-

specific activity

≥30 units/mg protein

concentration

20 mg/mL in Tris buffer

concentration

-

concentration

20-40 mg/mL

concentration

-

technique(s)

DNA purification: suitable

technique(s)

-

technique(s)

-

technique(s)

DNA purification: suitable

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute kits.

Application

RNase A solution has been used:

  • in apoptosis assay of Hep-2 cells
  • for the colorimetric quantification of methylated DNA
  • for DNA and RNA extraction
  • in DNA laddering analysis
  • in cell cycle analysis
  • as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Biochem/physiol Actions

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

Preparation Note

For usage requirements, please refer to the kit procedure.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

sample prep

Product No.
Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Homogeneous electrochemiluminescence biosensor for the detection of RNase A activity and its inhibitor
Jiancong N, et al.
Analytical Chemistry, 91, 14751-14756 (2019)
Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
Singh A, et al.
PloS one, 13(10), e0206667-e0206667 (2018)
Kevin P Foley et al.
Nature communications, 9(1), 4681-4681 (2018-11-10)
The intestinal microbiota and insulin sensitivity are rapidly altered after ingestion of obesogenic diets. We find that changes in the composition of the fecal microbiota precede changes in glucose tolerance when mice are fed obesogenic, low fiber, high fat diets
Phenotypic plasticity and biogeographic variation in physiology of habitat-forming seaweed: response to temperature and nitrate
Flukes EB, et al.
Journal of Phycology, 51(5), 896-909 (2015)
Abdou ElSharawy et al.
Oncotarget, 7(46), 75353-75365 (2016-09-30)
The emerging potential of miRNAs as biomarkers for cancer detection demands parallel evaluation of strategies for reliable identification of disease-related signatures from easily accessible and pertinent body compartments. Here, we addressed whether efficient concentration of circulating miRNA-carrying particles is a

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