R6501

Ribonuclease H from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous glycerol solution, 1,000-4,000 units/mL

• CAS Number: 9050-76-4
• Enzyme Commission number: 3.1.4.34 (BRENDA, IUBMB)
• MDL number: MFCD00132186
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: buffered aqueous glycerol solution
• mol wt: 17.6 kDa
• packaging: vial of ~30 units
• concentration: 1,000-4,000 units/mL
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Ribonuclease H from Escherichia coli has been used in a study to assess metallobiochemistry of the magnesium ion. Ribonuclease H has also been used in a study to investigate selective inhibitors of HIV-1 reverse transcriptase associated Rnase H activity.

Unit Definition

One unit hydrolyzes 1.0 nanomole RNA in ³H-labeled poly(A) • poly(dT) to acid soluble material in 20 min at 37 °C.

Physical form

Solution in 50% glycerol containing 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 10 mM MgCl₂, 0.1 mM EDTA, 0.1 mM DTT and 0.05 mg BSA per ml

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable