R7884

Ribonuclease B from bovine pancreas

BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)

• Synonym(s): RNase B
• CAS Number: 9001-99-4
• Enzyme Commission number: 3.1.27.5 (BRENDA, IUBMB)
• EC Number: 232-646-6
• MDL number: MFCD00132184
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 200
• assay: ≥80% (SDS-PAGE)
• form: powder
• specific activity: ≥50 Kunitz units/mg protein
• foreign activity: protease ≤0.001 units/mg solid
• storage temp.: −20°C

Description

Application

Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.

Packaging

500 μg in poly bottle

5, 10, 100, 500 mg

Package size based on protein content

Biochem/physiol Actions

Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.

Preparation Note

Purified by affinity chromatography

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves