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RAB0370

Sigma-Aldrich

Human MMP-8 ELISA Kit

for serum, plasma, conditioned medium, urine

Synonym(s):
MMP-8, Matrix metalloproteinase-8
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type urine
sample type conditioned medium

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MMP8(4317)

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RAB0361RAB0372RAB0362
packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type urine
sample type conditioned medium

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

assay range

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 24.69-18000 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human MMP-8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-8 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MMP8

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
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25G
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705578-5MG-PW

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Maria Bertolotto et al.
British journal of pharmacology, 171(14), 3376-3393 (2014-03-07)
Non-steroidal anti-inflammatory drugs (NSAIDs) have been shown to induce PG-independent anti-inflammatory actions. Here, we investigated the role of three different NSAIDs (naproxen, ibuprofen and oxaprozin) on neutrophil responses to CXCL8 and C5a. Human neutrophils were isolated from healthy volunteers by

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