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SAE0094

Sigma-Aldrich

SAvPhire Monomeric Streptavidin

recombinant, expressed in E. coli, High Affinity

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Synonym(s):
mSA3

recombinant

expressed in E. coli

form

lyophilized powder

shipped in

wet ice

storage temp.

−20°C

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This Item
HPA058617SAB5300203HPA056004
form

lyophilized powder

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous glycerol solution

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Biochem/physiol Actions

SAvPhire Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.
The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:
  • Minimizing distribution or dynamics in the probing of biotinylated targets
  • Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
  • Easier access to desired target

This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.

Physical properties

Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag

Reconstitution

Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.
This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:

  • 1 mg vial: add 250 μL of water for a 4 mg/mL solution
  • 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution

The reconstituted product can be further diluted in water or PBS as needed.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SAvPhire is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Functional expression of monomeric streptavidin and fusion proteins in Escherichia coli: applications in flow cytometry and ELISA.
Kroetsch, A. et al.
Applied Microbiology and Biotechnology, 102, 10079-10089 (2018)
Expression and purification of soluble monomeric streptavidin in Escherichia coli
Demonte D, et al.
Applied Microbiology and Biotechnology, 98(14), 6285-6295 (2014)

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