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SM0431

Sigma-Aldrich

MISSION® shRNA Mouse Gene Family Set, Lentiviral Particles

Phosphatases

NACRES:
NA.51

Quality Level

product line

MISSION®

storage temp.

−70°C

Gene Information

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General description

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.
Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.

Other Notes

Number of Genes: 219, Number of Clones: 1086
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.
For a detailed listing of other available gene family sets, visit the gene family set website.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Michel L Tremblay et al.
Cell metabolism, 7(2), 101-103 (2008-02-06)
FoxO transcription factors contribute to cardiac muscle remodeling and insulin signaling, but how they link insulin resistance and maladaptive heart hypertrophy remains unknown. A new study by Ni et al. (2007) shows that sustained activation of FoxO1 or FoxO3 in...
Wiljan J A J Hendriks et al.
The FEBS journal, 275(5), 816-830 (2008-02-27)
Some 40-odd genes in mammals encode phosphotyrosine-specific, 'classical' protein tyrosine phosphatases. The generation of animal model systems and the study of various human disease states have begun to elucidate the important and diverse roles of protein tyrosine phosphatases in cellular...
Yixiao Zou et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(29), 10429-10439 (2015-07-24)
Axonal growth and neuronal rewiring facilitate functional recovery after spinal cord injury. Known interventions that promote neural repair remain limited in their functional efficacy. To understand genetic determinants of mammalian CNS axon regeneration, we completed an unbiased RNAi gene-silencing screen...
Courtney G Woods et al.
Toxicology and applied pharmacology, 238(1), 27-36 (2009-04-21)
Hypochlorous acid (HOCl) is potentially an important source of cellular oxidative stress. Human HOCl exposure can occur from chlorine gas inhalation or from endogenous sources of HOCl, such as respiratory burst by phagocytes. Transcription factor Nrf2 is a key regulator...
Joshua P Klopper et al.
Molecular cancer, 8, 16-16 (2009-03-10)
Metastatic melanoma has a high mortality rate and suboptimal therapeutic options. Molecular targeting may be beneficial using the rexinoid LGD1069, a retinoid x receptor selective agonist, and thiazolidinediones (TZD), PPARgamma selective ligands, as novel treatments. Mouse xenograft models with human...

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