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SM2031

Sigma-Aldrich

MISSION® shRNA Mouse Gene Family Set, Lentiviral Particles

Tyrosine Kinases

NACRES:
NA.51

Quality Level

product line

MISSION®

storage temp.

−70°C

Gene Information

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General description

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.
Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.

Other Notes

The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.
Number of Genes: 133, Number of Cones: 850
For a detailed listing of other available gene family sets, visit the gene family set website.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Cecilia Annerén
Clinical science (London, England : 1979), 115(2), 43-55 (2008-06-13)
Pluripotent ES (embryonic stem) cells can be expanded in culture and induced to differentiate into a wide range of cell types. Self-renewal of ES cells involves proliferation with concomitant suppression of differentiation. Some critical and conserved pathways regulating self-renewal in...
Paolo M Comoglio et al.
Nature reviews. Drug discovery, 7(6), 504-516 (2008-05-31)
The MET tyrosine kinase stimulates cell scattering, invasion, protection from apoptosis and angiogenesis, thereby acting as a powerful expedient for cancer dissemination. MET can also be genetically selected for the long-term maintenance of the primary transformed phenotype, and some tumours...
Joshua P Klopper et al.
Molecular cancer, 8, 16-16 (2009-03-10)
Metastatic melanoma has a high mortality rate and suboptimal therapeutic options. Molecular targeting may be beneficial using the rexinoid LGD1069, a retinoid x receptor selective agonist, and thiazolidinediones (TZD), PPARgamma selective ligands, as novel treatments. Mouse xenograft models with human...
Courtney G Woods et al.
Toxicology and applied pharmacology, 238(1), 27-36 (2009-04-21)
Hypochlorous acid (HOCl) is potentially an important source of cellular oxidative stress. Human HOCl exposure can occur from chlorine gas inhalation or from endogenous sources of HOCl, such as respiratory burst by phagocytes. Transcription factor Nrf2 is a key regulator...

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