SRP0408

Histone Octamer full length human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 113.8 kDa
• packaging: pkg of 100 μg
• NCBI accession no.: NM_003528; NM_003532; NM_003548; NM_033445
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HIST1H2BG(8339) , HIST2H2AC(8338) , HIST3H3(8290) , HIST4H4(121504)

Description

General description

Human recombinant histone octamer consisting of 2 molecules each of histones H2A (GenBank Accession No. NM_033445) amino acids 2-130(end) with a N-terminal His-tag, H2B (GenBank Accession No. NM_003528) amino acids 2-126(end) with a N-terminal His-tag, H3 (GenBank Accession No. NM_003532) amino acids 2-137(end) with a N-terminal His-tag, and H4 (GenBank Accession No. NM_003548) amino acids 2-103(end) with a N-terminal His-tag, expressed in an E. coli expression system.

Packaging

100 μg

Biochem/physiol Actions

The nuclear DNA in eukaryotes is found to be associated with histones to form a compact complex called nucleosome. Histones neutralize the electrostatic nature of DNA and function as scaffolding proteins. Each core nucleosome contains two copies each of the core histones H2A, H2B, H3, and H4 to form an octameric complex. This octameric complex contains a central (H3-H4)₂ tetramer flanked on both sides with H2A-H2B dimers. The octamer complex function in various stages of chromosome function, chromatin assembly and nucleosome formation. The histone dimer-tetramer interactions are also important in RNA transcription.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable