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SRP0433

Sigma-Aldrich

PDE5A1 active murine

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

Synonym(s):
CN5N, phosphodiesterase 5A, cGMP-specific
NACRES:
NA.32

biological source

mouse

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

124 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

0.34 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... PDE5A(242202)

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SRP0436SRP0426SRP0262
PDE5A1 active murine recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0433

PDE5A1 active murine

PDE7A active rat recombinant, expressed in baculovirus infected Sf9 cells, ≥30% (SDS-PAGE)

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SRP0436

PDE7A active rat

PDE1B active rat recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

Sigma-Aldrich

SRP0426

PDE1B active rat

Sigma-Aldrich

Sigma-Aldrich

SRP0262

PDE4A1A Active human

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

assay

≥70% (SDS-PAGE)

assay

≥30% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

mol wt

124 kDa

mol wt

65 kDa

mol wt

89 kDa

mol wt

100 kDa

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

storage condition

-

General description

Mouse PDE5A (GenBank Accession No. NM_153422) full length with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Anindita Das et al.
The Journal of biological chemistry, 280(13), 12944-12955 (2005-01-26)
We investigated the effect of sildenafil in protection against necrosis or apoptosis in cardiomyocytes. Adult mouse ventricular myocytes were treated with sildenafil (1 or 10 microM) for 1 h before 40 min of simulated ischemia (SI). Necrosis was determined by
Hunter C Champion et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(5), 1661-1666 (2005-01-26)
The molecular mechanism for priapism is not well characterized. Although the nitric oxide (NO) pathway is known to mediate penile erection under normal conditions, we hypothesized that the mechanism of priapism rests in aberrant downstream signaling of this pathway based

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