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SRP3045

Sigma-Aldrich

Follistatin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
FS, activins-binding protein
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

0.1-0.4 μg/mL

mol wt

31.5 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FST(10468)

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F1175SAB1406997SRP3162
Sigma-Aldrich

Sigma-Aldrich

SRP3045

Follistatin human

Sigma-Aldrich

Sigma-Aldrich

F1175

Follistatin 300 human

recombinant

expressed in E. coli

recombinant

expressed in Sf21 cells

recombinant

-

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized

potency

0.1-0.4 μg/mL

potency

0.1-0.4 mg per mL

potency

-

potency

0.25 ng/mL ED50

mol wt

31.5 kDa

mol wt

31 kDa

mol wt

antigen ~38 kDa

mol wt

18.3 kDa

General description

Follistatin is a secreted protein that binds to ligands of the TGF (transforming growth factor)-β family and regulates their activity by inhibiting their access to signaling receptors. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. The gene is mapped to human chromosome 5q11.2. Recombinant human Follistatin is a 31.5kDa protein containing 288 amino acids. Its primary structure contains three cysteine-rich domains (called FS domains), each followed by a protease-inhibitory kazal domain.

Application

Follistatin human has been used to suppress myostatin signaling. It has also been used as an inhibitor of activin A.

Biochem/physiol Actions

Follistatin is a multifunctional protein. It was originally discovered as activin antagonists whose activity suppresses expression and secretion of the pituitary hormone FSH (follicle stimulating hormone). In addition to being a natural antagonist, follistatin can inhibit the activity of other TGF (transforming growth factor)-β ligands including BMP (bone morphogenetic protein)-2,-4,-6,-7, Myostatin, GDF-11 (growth differentiation factor 11), and TGF-b1. In gonads, it is responsible for oocyte competence and embryo development. In the pituitary gland, it causes cyclic changes in FSH production in the estrous cycle. Follistatin also participates in bone metabolism. It controls inflammation, fibrosis and tissue repair. Levels of plasma follistatin increases in polycystic ovary syndrome and type 2 diabetes. Follistatin might also be involved in tumor progression, including apoptosis, angiogenesis, and metastasis.

Sequence

GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN

Physical form

Lyophilized from 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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S Inouye et al.
Endocrinology, 129(2), 815-822 (1991-08-01)
Follistatin is a glycosylated monomeric protein originally isolated from ovarian follicular fluid based on its ability to specifically inhibit pituitary FSH release. To further explore the physiological role of follistatin, we have expressed recombinant human follistatins with 315 (rhFS-315) and
Ashlee W Harris et al.
Neuromuscular disorders : NMD, 25(9), 699-705 (2015-06-10)
Spinal muscular atrophy (SMA), a leading genetic cause of pediatric death in the world, is an early-onset disease affecting the motor neurons in the anterior horn of the spinal cord. This degeneration of motor neurons leads to loss of muscle
Regulation of ovarian function by the TGF-beta superfamily and follistatin.
Lin SY, et al.
Reproduction (Cambridge, England), 126, 133-148 (2003)
Myostatin knockout drives browning of white adipose tissue through activating the AMPK-PGC1a-Fndc5 pathway in muscle.
Shan T, et al.
Faseb Journal, 27, 1981-1989 (2013)
Serum Levels of Follistatin Are Positively Associated With Serum-Free Thyroxine Levels in Patients With Hyperthyroidism or Euthyroidism.
Tseng FY, et al.
Medicine, 95, e2661-e2661 (2016)

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