Merck
All Photos(1)

Documents

SRP3069

Sigma-Aldrich

IGF-I human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
IGF-IA, Somatamedin C
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥95% (HPLC)

form

lyophilized powder

potency

0.1-10 ng/mL ED50/EC50

mol wt

~7.6 kDa

packaging

pkg of 100 μg
pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IGF1(3479)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
I3769SRP3070GF306
Sigma-Aldrich

Sigma-Aldrich

SRP3069

IGF-I human

Sigma-Aldrich

Sigma-Aldrich

SRP3070

IGF-II human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

-

mol wt

~7.6 kDa

mol wt

~7.6 kDa

mol wt

7.5 kDa

mol wt

-

packaging

pkg of 100 μg, pkg of 50 μg

packaging

pkg of 50 and 100 μg

packaging

pkg of 50 μg

packaging

-

General description

IGFs (insulin like growth factors) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-I expression is regulated by growth hormone. IGF-I signal through the tyrosine kinase type I receptor (IGF-IR). Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.

Application

IGF-I human has been used:
  • as positive control in the quantification of growth factors in adipose-derived stem cells using enzyme-linked immunosorbent assay (ELISA)
  • as a medium supplement for trunk crestosphere cultures
  • in stimulation protein kinase B (Akt) methylation in human embryonic kidney cells (HEK293) and human melanoma cell line(A375)

In broiler chicks, IGF-I human has been used to study the effect of fadrozole and IGF-1 on female-to-male sex reversal and body weight.

Biochem/physiol Actions

The IGFs (insulin like growth factors) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF1 is an essential component for embryonic and postnatal skeletal development. Absence of IGF1 in mice negatively affects bone development, causes hypomineralization of skeletons and growth plate disturbances. It is also associated with cancer progression. It can suppress cell death and provides resistance to chemotherapy as well as radiation therapy.

Sequence

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Physical form

Lyophilized with no additives.

Reconstitution

It is recommended to centrifuge the vial prior to reconstitution. A stock solution may be prepared by reconstituting in sterile 0.2% acetic acid (recommended) to a concentration of 1.0 mg/ml. The stock solution may be diluted further using aqueous buffers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

J L Anderson et al.
Journal of dairy science, 98(8), 5709-5719 (2015-06-16)
The objective of this study was to determine if increased dietary fat from dried distillers grains with solubles (DDGS) in diets of growing heifers affected metabolic profile, plasma fatty acid profile, and reproductive maturation. Thirty-three Holstein heifers (133±18 d of
Sustained IGF-1 secretion by adipose-derived stem cells improves infarcted heart function
Bagno LL, et al.
Cell Transplantation, 25(9), 1609-1622 (2016)
Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides.
Rotwein P
The Journal of Biological Chemistry, 261, 4828-4832 (1986)
Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres
Mohlin S, et al.
Developmental Biology, 447(2), 137-146 (2019)
AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions
Guo J, et al.
Nature Cell Biology, 21(2), 226-226 (2019)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service