SRP3177

TNF-α human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): Cachectin, Cytotoxin, Differentiation-inducing factor (DIF), Necrosin, Tumor Necrosis Factor
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 300
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: ≤0.10 ng/mL ED₅₀
• mol wt: 17.4 kDa
• packaging: pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.00 EU/μg endotoxin
• UniProt accession no.: P01375
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TNF(7124)

Description

General description

TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts.[1][2] It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2.[3] The gene is mapped to human chromosome 6p21.3.[4] Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Application

TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines.[4]

Biochem/physiol Actions

TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor.[3][2] TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes.[5][6][7][8][9] It is also associated with mood and anxiety disorders.[10]

Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.

Reconstitution

It is recommended to centrifuge the vial beforereconstitution. A stock solution may be prepared byreconstitution in sterile water to a concentration of0.1 mg/mL. The stock solution may be diluted furtherusing culture media.

Storage and Stability

Store the product at −20 °C. After reconstitution, theproduct can be stored at 2-8 °C for up to 1 month.For extended storage, freeze in working aliquots.Repeated freezing and thawing are not recommended.Do not store in a frost-free freezer.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable