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SRP3268

Sigma-Aldrich

PDGF-AA human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

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Synonym(s):
GDGF, ODGF
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-3.0 ng/mL ED50

mol wt

28.5 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PDGFA(5154)

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Sigma-Aldrich

Sigma-Aldrich

SRP3268

PDGF-AA human

Sigma-Aldrich

Sigma-Aldrich

SRP3138

PDGF-BB human

Sigma-Aldrich

Sigma-Aldrich

SRP3229

PDGF-BB from mouse

Sigma-Aldrich

Sigma-Aldrich

SRP3228

PDGF-AA from mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

mol wt

28.5 kDa

mol wt

24.3 kDa

mol wt

24.4 kDa

mol wt

28.7 kDa

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

General description

Platelet derived growth factors (PDGFs) are disulfide-linked glycoprotein dimers consisting of two 12.0-13.5kDa polypeptide chains, designated as PDGF-A and PDGF-B chains. There are three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kDa disulfide-linked homodimer of two A chains (250 total amino acids).

Biochem/physiol Actions

The platelet derived growth factors (PDGFs) are stored in platelet a-granules and are released upon platelet activation. They are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. These proteins are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells.

Physical form

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Characterization of the G-quadruplexes in the duplex nuclease hypersensitive element of the PDGF-A promoter and modulation of PDGF-A promoter activity by TMPyP4.
Qin Y
Nucleic Acids Research, 35(22), 7698-7713 (2007)
The use of autologous blood-derived growth factors in bone regeneration.
Civinini R
Clinical Cases in Mineral and Bone Metabolism : The Official Journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 8(1), 25-31 (2011)
Brashket Seth et al.
Neurotoxicology, 70, 161-179 (2018-11-25)
During the mammalian brain development, oligodendrocyte progenitor cells (OPCs) are generated from neuroepithelium and migrate throughout the brain. Myelination is a tightly regulated process which involves time framed sequential events of OPCs proliferation, migration, differentiation and interaction with axons for
Structural and functional studies on platelet-derived growth factor.
C H Heldin
The EMBO journal, 11(12), 4251-4259 (1992-12-01)
The biology of platelet-derived growth factor.
R Ross et al.
Cell, 46(2), 155-169 (1986-07-18)

Articles

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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