SRP3269

RANTES human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): CCL5, SIS-delta
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 1.0-10.0 ng/mL
• mol wt: 7.8 kDa
• packaging: pkg of 20 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: P13501
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CCL5(6352)

Description

General description

Regulated on activation, normal T cell expressed and secreted (RANTES) also known as C-C motif chemokine ligand 5 (CCL5) is encoded by the gene mapped to human chromosome 17. RANTES belongs to intercrine or chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections. Recombinant human RANTES is a 7.8kDa protein containing 68 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Application

RANTES human has been used in following studies:

• In transmigration experiments to determine whether LFA-1 cross-linking could affect peripheral blood T lymphocytes (PBTL) migration in response to the chemokine RANTES.
• Luminol-dependent chemiluminescence assay to determine the effect of RANTES on reactive oxygen species (ROS) production by eosinophils.

Biochem/physiol Actions

Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4⁺/CD45RO), basophils, and eosinophils. The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5.1 RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. The In1.1C variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV-1 infection.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable