SRP6238

TRAIL murine

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): Apo2 Ligand, TL2, TNF-related apoptosis-inducing Ligand, TNFSF10
• NACRES: NA.32

Properties

• biological source: rat
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 20 kDa
• packaging: pkg of 10 μg; pkg of 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P50592
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... TRAIL(22035)

Description

General description

TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which activates the cell′s apoptotic machinery. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable