SRP6266

Renin from mouse

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CYM, CYMP, Chymosin, RENNIN
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: calculated mol wt 43.2 kDa; observed mol wt 45-55 kDa by SDS-PAGE (reducing) (Protein migrates due to glycosylation. Leu24 is the predicted N-terminus.)
• packaging: pkg of 10 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P06281
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... REN(19701)

Description

General description

Renin, also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body′s renin-angiotensin system (RAS). The gene encoding it is localized on mouse chromosome 1.

Biochem/physiol Actions

Renin plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. It activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by angiotensin-converting enzyme (ACE), the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through β-1 receptor activation. It can bind to ATPase H⁺ transporting accessory protein 2 (ATP6AP2), which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, it′s binding results in phosphorylation of serine and tyrosine residues of ATP6AP2. The level of renin mRNA appears to be modulated by the binding of hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), β subunit (HADHB), HuR and CP1 to a regulatory region in the 3′ UTR. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable