Merck
  • T5694
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T5694

Sigma-Aldrich

TRAIL human

recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder

All Photos(1)
Synonym(s):
Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand
CAS Number:
2244730-93-4
MDL number:
MFCD01867158
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in NSO cells

description

N-terminal 6X histidine-tagged extracellular domain

Assay

>97% (SDS-PAGE)

form

lyophilized powder

mol wt

24 kDa by SDS-PAGE
predicted mol wt ~21 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P50591

storage temp.

−20°C

Gene Information

human ... TNFSF10(8743)

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TRAIL human

TRAIL, Soluble human recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

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TRAIL, Soluble human

biological source

human

biological source

rat

biological source

human

biological source

human

recombinant

expressed in NSO cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE and HPLC)

assay

>98% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

liquid

mol wt

24 kDa by SDS-PAGE, predicted mol wt ~21 kDa

mol wt

20 kDa

mol wt

19.6 kDa, predicted mol wt ~21 kDa

mol wt

~26 kDa

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

immunofluorescence: suitable

technique(s)

activity assay: suitable, cell culture | mammalian: suitable, transfection: suitable

Application

TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.

Biochem/physiol Actions

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.
Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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