Merck
All Photos(1)

T9069

Sigma-Aldrich

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

Synonym(s):
DNA Topoisomerase I
Enzyme Commission number:
MDL number:
NACRES:
NA.54

recombinant

expressed in baculovirus

Quality Level

form

buffered aqueous glycerol solution

mol wt

100 kDa

concentration

≥2 units/μL

UniProt accession no.

storage temp.

−70°C

Gene Information

human ... TOP1(7150)

General description

Topoisomerase I (TOP1) is mapped to human chromosome 20q12.[1] TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.[2]

Application

Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. [3] Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells. [4]
Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.[5]

Biochem/physiol Actions

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation.[2] TOPI is essential for normal development and transcriptional regulation.[6] Variants in the TOP1 gene is observed in patients with colorectal cancer.[1]
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

Unit Definition

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

Physical form

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service