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SygRNA® Cas9 Synthetic tracrRNA

SygRNA® SpCas9 tracrRNA


pkg of 5 nmol × Tube (HPLC)

shipped in


storage temp.


General description

The SygRNA® synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA® synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.


Functional Genomics/Transgenic Applications
  • Creation of gene knockouts
  • Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes

Features and Benefits

HPLC-purified SygRNA® Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.


The SygRNA® Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.

Learn more about SygRNA® tracrRNA

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Physical form


Preparation Note

Resuspend SygRNA® Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

Legal Information

SygRNA is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Manuel Garcia-Moreno et al.
Molecular cell, 74(1), 196-211 (2019-02-26)
The compendium of RNA-binding proteins (RBPs) has been greatly expanded by the development of RNA-interactome capture (RIC). However, it remained unknown if the complement of RBPs changes in response to environmental perturbations and whether these rearrangements are important. To answer these


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