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Anti-Calbindin D-28K clone 1A11 Antibody, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Calbindin, D-28K, D28, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein avian-type

biological source

rabbit (recombinant)


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


1A11, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more



mol wt

calculated mol wt 29.99 kDa
observed mol wt ~28 kDa

species reactivity

mouse, human, rat


antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


immunocytochemistry: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400
western blot: suitable using 1:1,000



UniProt accession no.

greener alternative category

shipped in


storage temp.


Gene Information

mouse ... Calb1(12307)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 1A11 is a ZooMAb rabbit recombinant monoclonal antibody that detects Calbindin. It targets an epitope with in the C-terminal half.


GST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal half of murine Calbindin.


Anti-Calbindin D-28K, clone 1A11, ZooMAb, Cat. No. ZRB1778, is a recombinant Rabbit Monoclonal Antibody that detects Calbindin D-28K and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Calbindin D-28K in mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Calbindin D-28K in mouse kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calbindin D-28K in PC12 cells.


25, 25 μL

Target description

Calbindin (UniProt: P12658; also known as Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type) is encoded by the calb1 gene (gene ID: 12307) in murine species. Calbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. Calbindin D-28K has four functional calcium-binding sites (aa 24-35; 111-122; 155-166; and 199-210). It has moderate cytoplasmic mobility and comprises six EF-hands, of which one or two are non-functioning in metal binding. EF-2 is viewed as non-metal binding and EF-6 may have a very low Ca2+ affinity. Four EF hands function in calcium binding with rapid to intermediate kinetics and affinity. The biological roles of Calbindin D-28K include calcium regulation and signaling, including calcium transport and uptake, calcification of bone and teeth, and calcium related signaling in neurons and transiently in embryological development. It also protects neurons from apoptotic cell death. Decline in Calbindin D-28K levels in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28K immunoreactivity is shown to be specifically absent from neuronal populations lost early in ALS, while substantial loss of neurons, which do contain this protein occur in patients with Huntington′s disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Schmidt, H (2012). Front. Mol. Neurosci. 5; 25).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids



Certificate of Analysis

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Certificate of Origin

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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