RNAi Single Clones

Stylized hairpin DNA

MISSION® shRNA Clones for Optimal Gene Knockdown 

Long term, stable gene silencing distinguishes short hairpin RNA constructs (shRNA) from other RNAi and CRISPR tools and can offer distinct advantages over knockout gene experiments. Sigma-Aldrich® Advanced Genomics, in collaboration with the Broad Institute’s RNAi consortium (TRC) scientists, have generated the most comprehensive and validated series of vectors and clones for your RNAi experiments. Take advantage of our world-class expertise in high-throughput lentiviral manufacturing and DNA purification services, which can save you time and resources. Flexible customization offerings ensure all your experimental needs are met and offers unparalleled gene coverage.  

With over 350 years of technical leadership, Sigma-Aldrich® Advanced Genomics products come with expertise that extends Beyond the Bench—from discovery to therapeutic impact.

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Expert Gene Knockdown You Can Count On

As the exclusive provider of the trusted TRC1.5 and TRC2 collections, our comprehensive MISSION® shRNA product offering consists of almost 200,000 pre-cloned shRNA constructs targeting more than 20,000 human and mouse genes. Easily search by your gene of interest, view validation data, and order in a few simple clicks. shRNA clones are available in lentivirus, plasmid DNA, or bacterial stocks.

Each gene is represented by multiple vector constructs targeting different regions of the gene sequence. Every shRNA is cloned into the pLKO.1 lentiviral vector, and is sequence verified to ensure a match to the target gene. Sigma-Aldrich® Advanced Genomics offers the most trusted and comprehensive shRNA screening portfolio for even the most difficult targets and cell lines.

RNAi Screening for Genome-Wide Knockdown

Functional genomics screens are an integral part of discovery research, allowing scientists to unravel the mechanisms underlying biological systems, pathways, and diseases. Effective interrogation of these complex molecular interaction networks requires shRNA libraries that deliver reliable, efficient, and long-term knockdown. Need more info on gene family sets or libraries? Contact Us.


Optimized experimental designs start with the right vector and controls. Shop our comprehensive offering of vector options and positive and negative controls for TRC1.5 and TRC2 available in plasmid DNA or lentiviral transduction particles. Learn more about MISSION® shRNA controls. Learn more about MISSION® vectors.

Custom Cloning

Whether you need a vector with a fluorophore tag, alternate promoters, or higher viral titers, our collection has you covered. Our primary backbones are the TRC-designed pLKO.1-puro and TRC2-pLKO.5-puro vectors. These vectors can be adapted to meet your needs for any experiment.

Have a custom project you want to discuss? Connect with a Sigma-Aldrich® Advanced Genomics Expert.

MISSION® shRNA Knockdown Guarantee

Sigma-Aldrich® shRNA is guaranteed that upon purchase of the defined lentiviral clone set quantity on the shRNA product page for your gene of interest (this varies, but is typically 5 clones), at least one of those lentiviral clones for a gene should yield greater than 70% knockdown.
This guarantee does not apply to transient transfections with DNA. Proper controls and conditions are required to go along with supporting data showing ineffective knock-down for the guarantee to apply. Appropriate supporting data would include quantitative PCR-based silencing data, comparing target mRNA levels of an appropriate positive control shRNA to an appropriate negative control shRNA, demonstrating knockdown of the target mRNA greater than or equal to 70%. Due to the variability of antibodies and protein half-lives, we are unable to accept protein-based detection methods. In the case that there are no more shRNA for that gene target, or all shRNA fail to knock down the target gene by 70% or more, we will refund the price for the clone set quantity.

We’re Here to Help

Whether you need help designing your experiment, need a custom vector, high viral titer, require assistance troubleshooting, or simply want to better understand how you can apply shRNA gene silencing in your research, Sigma-Aldrich® Advanced Genomics is here to guide you. Connect with a Sigma-Aldrich® Advanced Genomics Expert

Related Product Resources

  • Getting Started with MISSION® shRNA

    Gene silencing and knockdown using RNA interference is becoming routine. The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

  • MISSION shRNA Library: Next Generation RNA Interference

    Work and refinement of the RNAi technology has exploded in recent years. We now know the basic mechanism of the endogenous RNAi pathway, that the pathway is present in most eukaryotes,3 and how cellular machinery can be harnessed to silence gene expression.

  • MISSION® Application Data

    MISSION® Application Data. We are committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries.


    We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.

  • Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

    Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways

  • Brochure: MISSION® RNAi Tools

    Depend on our comprehensive shRNA & miRNA portfolio.

  • Vector Maps

    A series of vectors to enable a successful implementation of your RNAi experiments.

  • General shRNA FAQs

    We answers your frequently asked questions about shRNA, the TRC. MISSION and more.

  • Lentiviral Transduction Protocol

    Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

  • Advanced RNAi Applications — Difficult Cell Types FAQs

    We answer your frequently asked questions about using shRNA with difficult cell types.

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